Pewarnaan Asid Nukleik RedView
RedView Nucleic Acid Stain is a fluorescent dye for post-electrophoresis staining of DNA and RNA. It is formulated as a safe, high-sensitivity replacement for ethidium bromide (EtBr). RedView provides stronger signals than EtBr with similar excitation/emission characteristics, making it compatible with standard UV transilluminators and imaging systems.
Features of RedView Nucleic Acid Stain
- EtBr Replacement: Sensitive, stable fluorescence dye designed to substitute toxic ethidium bromide.
- Non-Toxic & Non-Mutagenic: Ames-tested at working dilutions and confirmed free of mutagenicity, offering a safer alternative for lab personnel.
- High Sensitivity: Enhanced detection of dsDNA, ssDNA and RNA, providing stronger signals than EtBr.
- Spectral Compatibility: Excitation/emission spectrum similar to EtBr, fully compatible with existing UV transilluminators and imaging equipment.
- Cost-Effective: Supplied as a 10,000× stock solution, enabling economical use (one vial stains over 100 gels).
Description of RedView Nucleic Acid Stain
RedView can be used by adding the stain to molten agarose for precasting or by post-staining gels after electrophoresis. A single 10,000× stock of RedView provides enough reagent for at least 100 mini-gels. Importantly, DNA recovered from a RedView-stained gel is virtually free of dye by phenol/chloroform extraction and ethanol precipitation, ensuring compatibility with downstream applications. RedView has been Ames-tested at working concentrations and is confirmed to be non-mutagenic and non-carcinogenic.
Applications of RedView Nucleic Acid Stain
- Staining of dsDNA, ssDNA, and RNA.
- Use in agarose and polyacrylamide gel electrophoresis.
- Staining using either the gel staining method (stain before electrophoresis) or the soaking staining method (stain after electrophoresis).
Why Choose Our RedView Nucleic Acid Stain?
- Safety Profile: It is a safe, non-toxic nucleic acid dye. It is a unique lipophilic macromolecule that does not penetrate cell membranes to enter cells. Furthermore, it is non-volatile, non-sublimable, and not inhaled by humans.
- Spectral Properties & Compatibility: It has the same spectral properties as ethidium bromide (EB). It can be detected under ultraviolet (UV) or blue light excitation without needing to change filters or viewing equipment (compatible with common UV gel imagers).
- Ease of Use: It has good thermal stability and can be added directly to hot agarose solutions, and compatible with all commonly used electrophoresis buffer solutions.
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